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Gene expression analysis by reverse transcriptase-quantitative real-time PCR (RT-qPCR) After the homogenization step by using a TissueLyser II (Qiagen, Hilden, Germany) in the gastric tissue, total RNA was isolated using a commercial kit (12183018A PureLink RNA Mini Kit, Thermo Fisher) in accordance with the manufacturers recommendations

PubMed Central , The FASEB Journal , vol

Bioavailability refers to the amount of a substance that actually enters the bloodstream and becomes available for use by the body

Table (3): ADME validation of the metabolites from Burkholderia stagnalis Selected bioactive metabolites obeyed Lipinskis rule of 5 without any violation suggesting their possibility of oral administration, The boiled egg plot evaluate the pharmacokinetic properties of the 24 bioactive metabolites, helping to predict their bioavailability and potential therapeutic applications (Figure 6)